Simultaneous determination of psuedoephedrine, fexofenadine and loratadine in pharmaceutical products using high resolution RP-HPLC method
ABSTRACTHigh resolution RP-HPLC method has been developed for the simultaneous estimation of Psuedoephedrine, Fexofenadine and Loratadine in pharmaceutical products. Separation was achievedby using C18, 150 x 4.6 mm, 5μm column at a temperature of 350C with a gradient mobile phase composed of sol-A: 0.01M pH 3.0 phosphate (KH2PO4) buffer, sol-B: Acetonitrile and gradientprogram was 0-5min, sol-A: 80-60; 5-10min- sol-A: 60-20; 10-15min- sol-A: 20-20; 15-17min- sol-A: 20-80 and 17-20min- sol-A: 80-80. Flow rate was 0.60 ml per min and measured theabsorbance at 210nm. The retention time of…