New cells develop in the heart but how these cardiac cells are born and how frequently they are generated remains unclear. New research from Brigham and Women's Hospital used a novel method to identify these new heart cells and describe their origins - a Multi-isotope Imaging Mass Spectrometry (MIMS) imaging system that demonstrates cell division in the adult mammalian heart.
The researchers marked existing cardiac cells genetically to cause them to express a green fluorescent protein. Then they used Multi-isotope Imaging Mass Spectrometry to examine the development of new heart muscle cells - cardiomyocytes - in a pre-clinical model over a period of months. Researchers said they were surprised to find that new heart muscle cells primarily arose from existing heart muscle cells, rather than stem cells. Even in the setting of a heart attack, when stem cells are thought to be activated, most new heart cells were born from preexisting heart cells.
"The question about how often cardiac cells are born has been extremely difficult to answer because there was a need for new techniques to help us understand this process. We are especially excited about our findings because of the novel way in which were able to show new heart cells, using Multi-isotope Imaging Mass Spectrometry (MIMS). Our collaborator, Claude Lechene, MD, had developed this technology, and as a team we harnessed this for the cardiac regeneration question." said Richard T. Lee, MD, physician, researcher in the Cardiovascular Division at Brigham and Women's Hospital and senior author of the paper. "These data present one piece of the puzzle when it comes to the discussion around the generation of new cardiac cells.
"Our data show that adult cardiomyocytes are primarily responsible for the generation of new cardiomyocytes and that as we age, we lose some capacity to form new heart cells," said Dr. Lee. "This means that we are losing our potential to rebuild the heart in the latter half of life, just when most heart disease hits us. If we can unravel why this occurs, we may be able to unleash some heart regeneration potential."
Published in Nature.
