HoxD13: Researchers Engineer Zebrafish To Grow A Leg

Neil Shubin alert: Researchers have found evidence that the development of hands and feet occurred through the gain of new DNA elements that activate particular genes - and they used a zebrafish to show it. The transition from water to land was obviously a major event in the history of vertebrate life. In order to understand how fins may have evolved into limbs, researchers introduced extra Hoxd13, a gene known to play a role in distinguishing body parts, at the tip of a zebrafish embryo's fin. This led to the generation of new cartilage tissue and the reduction of fin tissue—changes that are similar to key aspects of land-animal limb development.

The transition from water to land was obviously a major event in the history of vertebrate
life. In order to understand how fins may have evolved into limbs, researchers introduced extra Hoxd13, a gene known to play a role in distinguishing body parts, at the tip of a zebrafish embryo's fin. This led to the generation of new cartilage tissue and the reduction of fin tissue—changes that are similar to key aspects of land-animal limb development.

The researchers wondered whether novel Hoxd13 control elements may have increased Hoxd13 gene expression in the past to cause similar effects during limb evolution. They turned to a DNA control element that is known to regulate the activation of Hoxd13 in mouse embryonic limbs and that is absent in fish.

This zebrafish embryo developed a limb that looks more like a leg than a fin, after being engineered to produce the HoxD13 protein within the cells at the tip of the developing fin. Changes in HoxD13 production likely contributed to the transition from fin to leg development, during animal evolution. Credit: Freitas et al., Developmental Cell

"First, and foremost, this finding helps us to understand the power that the modification of gene expression has on shaping our bodies," says Dr. José Luis Gómez-Skarmeta of the CSIC-Universidad Pablo de Olavide-Junta de Andalucía, in Seville, Spain. "Second, many genetic diseases are associated with a 'misshaping' of our organs during development. In the case of genes involved in limb formation, their abnormal function is associated with diseases such as synpolydactyly and hand-foot-genital syndrome."

"We found that in the zebrafish, the mouse Hoxd13 control element was capable of driving gene expression in the distal fin rudiment. This result indicates that molecular machinery capable of activating this control element was also present in the last common ancestor of finned and legged animals and is proven by its remnants in zebrafish," says Dr. Fernando Casares.

Published in Developmental Cell.

Old NID

98584